Research Paper Volume 13, Issue 23 pp 25393—25407

Functional rare variant in a C/EBP beta binding site in NINJ2 gene increases the risk of coronary artery disease


Figure 4. The risk allele A of rs34166160 has a higher transcriptional activity under the condition of LPS stimuli. Data from luciferase assays in HUVECs. The PGL-3-promoter luciferase vectors containing the 30 bp length genomic fragment overlapping rs34166160 harboring risk A allele or wide type C allele were transfect into HUVECs for 24 h, and then LPS (1 ug/ml) stimulated for another 24 h. The measurement of luciferase activities was previously described. Three independent experiments were performed. Error bars represent standard deviation (SD). *P < 0.05.