Figure 1. Differential expression profile of lncRNAs between AS and healthy patients. (A) The differential genes are represented by volcano plots. Gray points are genes with P values ≥ 0.05. Green point are genes with absolute fold changes ≥ 2 and P values < 0.05. The red dots are genes with fold changes ≥ 2 and P values < 0.05. (B) A heatmap showing differentially expressed lncRNAs. Green and red colors stand for down-regulation and up-regulation, respectively. (C) qRT-PCR was performed to detect the expression of lncRNAs in AS serum. (D) An ROC analysis of lncRNAs expression to differentiate between AS and healthy controls. Results are expressed as mean ± SD. *P < 0.05.