Research Paper Volume 13, Issue 23 pp 25408—25425

lncRNA CASC7 regulates pathological progression of ox-LDL-stimulated atherosclerotic cell models via sponging miR-21 and regulating PI3K/Akt and TLR4/NF-κB signaling pathways


Figure 3. CASC7 is a decoy of miR-21. (A) The differential genes are represented by volcano plots. Gray points are genes with P values ≥ 0.01. Green point are genes with absolute fold changes ≥1 and P values < 0.01. The red dots are genes with fold changes ≥1 and P values < 0.01. (B) A heatmap showing differentially expressed miRNAs. Green and red colors stand for down-regulation and up-regulation, respectively. (C) Intersection analysis of target miRNAs of CASC7 and differentially expressed miRNAs. (D) The binding site of CASC7 in the 3′-UTR of miR-21 was predicted by TargetScan. (E and F) Luciferase activity of CASC7 as detected by a dual-luciferase reporter assay. Results are expressed as mean ± SD. *P < 0.05.