Figure 4. miR-21 reverses the biological effects of CASC7 on VSMC and HUVEC. (A) Expression of miR-21 in VSMC as detected by qRT-PCR. (B) Cell viability evaluated by CCK-8 assays. (C and D). Apoptosis rates of VSMC were tested by flow cytometry. (E and F) Invasion capability was evaluated by Transwell invasion assays. (G) Expression of miR-21 in HUVEC as detected by qRT-PCR. (H) Levels of inflammatory factors, including TNF-α, IL-1β and IL-6, were detected by ELISA. Photomicrographs show representative images of the VSMC and HUVEC (400×). Data are presented as mean ± SD. *P < 0.05.