Research Paper Volume 13, Issue 23 pp 25408—25425

lncRNA CASC7 regulates pathological progression of ox-LDL-stimulated atherosclerotic cell models via sponging miR-21 and regulating PI3K/Akt and TLR4/NF-κB signaling pathways


Figure 5. The PI3K/Akt pathway is a target of miR-21. (A) The binding site of miR-21 in the 3′-UTR of PI3K was predicted by TargetScan. (B) Luciferase activity of PI3K as detected by dual-luciferase reporter assay. (CH) Expression of TLR4, NF-κB, PI3K and Akt examined by Western blot assays. (IL) Luciferase activity of PI3K and Akt in VSMC examined by immunofluorescence assays. Representative images in VSMC (400×). Data are presented as mean ± SD. *P < 0.05.