Figure 5. The PI3K/Akt pathway is a target of miR-21. (A) The binding site of miR-21 in the 3′-UTR of PI3K was predicted by TargetScan. (B) Luciferase activity of PI3K as detected by dual-luciferase reporter assay. (C–H) Expression of TLR4, NF-κB, PI3K and Akt examined by Western blot assays. (I–L) Luciferase activity of PI3K and Akt in VSMC examined by immunofluorescence assays. Representative images in VSMC (400×). Data are presented as mean ± SD. *P < 0.05.