Figure 6. The TLR4/NF-κB axis is a target of miR-21. (A) The binding site of miR-21 in the 3′-UTR of TLR4 was predicted by TargetScan. (B) Luciferase activity of TLR4 as detected by dual-luciferase reporter assay. (C–H) Expression of TLR4, NF-κB, PI3K and Akt examined by Western blot assays. (I–L) Luciferase activity of TLR4 and NF-κB in HUVEC examined by immunofluorescence assays. Representative images of HUVEC (400×). Data are presented as mean ± SD. *P < 0.05.