Research Paper Volume 14, Issue 1 pp 286—296
Yes-associated protein reacts differently in vascular smooth muscle cells under different intensities of mechanical stretch
Figure 4. Inhibition of the Rho-ROCK pathway affects the Hippo pathway under different intensities of stretch. (A) The YAP localization of VSMCs was detected by immunocytochemistry. (B) Quantification of nuclear YAP amount in VSMCs. (C) The levels of phosphorylated YAP, phosphorylated Lats and ccnd1 in VSMCs were detected by western blotting. Values are expressed as means±SD. **P < 0.01, compared with 0% elongation+siRNA(-) group; ##P < 0.01, compared with 10% elongation+siRNA(-) group; ^^P < 0.01, compared with 20% elongation+siRNA(-) group; @@P < 0.01, compared with 0% elongation+siRNA(+) group.