Figure 1. Circ_0008259 expression level is downregulated in OS. (A) The volcano plot showed that multiple circRNAs were up-regulated or down-regulated in OS tissues vs. non-cancerous tissues. Green plots represent down-regulated circRNAs, and red plots represent up-regulated circRNAs. (B) Circ_0008259 and linear LMO7 expression levels were detected by qRT-PCR after the total RNA was treated with RNase R. (C) Circ_0008259 was mainly presented in the cytoplasm of OS cells, which was verified by FISH. (D) qRT-PCR was used to detect the expression of circ_0008259 in OS tissues (n=50) and adjacent tissues (n=50). (E) qRT-PCR was used to detect the expression of circ_0008259 in normal osteoblast (hFOB1.19) and OS cell lines (143B, HOS, U2OS, and MG63). **P < 0.01, ***P < 0.001.