Figure 4. MiR-21-5p specifically regulates PDCD4. (A). StarBase database was used to predict the binding site between miR-21-5p and PDCD4. (B) Dual-luciferase reporter assay was used to confirm the interaction between miR-21-5p and PDCD4. (C, D) qRT-PCR and Western blot assays was used to detect the expression of PDCD4 after circ_0008259 and miR-21-5p were selectively regulated. (E) The expression of PDCD4 was negatively correlated with the expression of miR-21-5p in OS tissues (R2=0.4009, P<0.001). ***P < 0.001.