Figure 3. IGFBP-6 induces the expression of mediators involved in proliferation and migration in HS5 cells. (A–G) Multiplex immunobead assay technology on HS5 cells exposed to 200 ng/mL of IGFBP-6 for 24h and 48h was performed on a culture medium to determine concentrations of indicated cytokines. Culture medium from untreated cells and treated cells were evaluated. (*P < 0.05) (H) qPCR results obtained for BMP2 in HS5 cells exposed to 200 ng/mL of IGFBP-6 for 24h and 48h. Relative mRNA expression level normalized with β-actin by using a comparative 2-ΔΔCt method. *** P < 0.001.