Research Paper Volume 13, Issue 24 pp 26137—26147

miR-545 promotes colorectal cancer by inhibiting transferring in the non-normal ferroptosis signaling


Figure 4. miR-545 targets the transferrin gene. (A) A conserved miR-545 binding site was identified in the TF 3’UTR using TargetScan. (B) A dual luciferase reporter assay demonstrated that miR-545 significantly decreased the relative luciferase reporter activity of the pmirGLO-TF-3’UTR. A Western blot assay showed that overexpression of miR-545 significantly decreased TF expression in HT-29 and HCT-116 cells (C), but inhibition of miR-545 significantly increased TF expression (D). *p < 0.05, ***p < 0.001 vs. as indicated.