Research Paper Volume 14, Issue 1 pp 368—388

LINC02362 attenuates hepatocellular carcinoma progression through the miR-516b-5p/SOSC2 axis


Figure 10. SOCS2 mediates the inhibitory effects of LINC02362 on HCC cell survival. (A, B) RT-qPCR quantification (n=3) of LINC02362 (A) and SOCS2 (B) expression in Hep3B and PLC/PRF/5 cells. (C) Quantification of SOCS2 levels by western blotting (n=3) with LINC02362 and SOCS2 misexpression. (D) MTT assay (n=3) for measuring the proliferative abilities of HCC cells with LINC02362 and SOCS2 misexpression. (E, F) EdU labeling to detect the percentage of dividing cells in LINC02362 and SOCS2 misexpressing HCC cells (E) and the corresponding quantification (F; n=3). Bar=100μm. (G, H) Measurement (G) and quantification (H; n=3) of the cell cycle in HCC cells by flow cytometry. (I, J) Detection (I) and quantification (J; n=3) of apoptotic cells in HCC cells misexpressing LINC02362 and SOCS2. # 0.01 < P < 0.05, ** or ## 0.001 < P < 0.01, *** or ### 0.0001 < P < 0.001, **** P < 0.0001. NS, not significant.