Figure 4. LINC02362 functions as a sponge for miR-516b-5p. (A) Subcellular fractionation (n=3) for quantifying the localization of LINC02362 in HCC cells. (B) Venn diagram showing the overlap between the indicated three databases. (C) Schematic plot indicating the putative binding site between LINC02362 and miR-516b-5p and the sequences upon mutagenesis. (D) Dual luciferase assays (n=3) for checking the effects of miR-516b-5p on the indicated 3’UTR constructs. (E) RT-qPCR detection (n=3) of the miR-516b-5p expression upon misexpression of LINC02362. (F) Datamining to check the levels of miR-516b-5p in non-tumor (NT; n=50) or HCC tissues (n=370). (G) Scatter plot showing the correlation between LINC02362 and miR-516b-5p. (H, I) Kaplan-Meier plots showing the overall survival (OS, H) or disease-free survival (DFS, I) of HCC patients stratified by miR-516b-5p levels. **0.001 < P < 0.01, *** or ### 0.0001 < P < 0.001. NS, not significant.