Figure 7. miR-516b-5p is critical for the effects of LINC02362 on HCC cell survival. (A) RT-qPCR quantification of miR-516b-5p (left) and LINC02362 (right) expression in Hep3B and PLC/PRF/5 cells (n=3). (B) MTT assay (n=3) for measuring the proliferative abilities of HCC cells overexpressing miR-516b-5p and/or LINC02362. (C) EdU labeling to detect the percentage of dividing cells in LINC02362 and miR-516b-5p-overexpressing HCC cells (left) and the corresponding quantification (right; n=3). Bar=100μm. (D) Measurement (left) and quantification (right; n=3) of the cell cycle in HCC cells by flow cytometry. (E) Detection (left) and quantification (right; n=3) of apoptotic cells in HCC cells overexpressing LINC02362 and miR-516b-5p. ** 0.001 < P < 0.01, *** or ### 0.0001 < P < 0.001, **** or #### P < 0.0001.