Figure 9. SOCS2 is a downstream target gene of miR-516b-5p. (A) Venn diagram indicating the overlap between the indicated 5 databases. (B) Schematic plot expressing the putative binding site between miR-516b-5p and SOCS2 and the sequences upon mutagenesis. (C) Dual luciferase assays (n=3) for checking the effects of miR-516b-5p on the indicated 3’UTR constructs. (D, E) RT-qPCR (D) and western blotting (E) detection (n=3) of the SOCS2 expression upon misexpression of miR-516b-5p. (F) Scatter plot showing the correlation between miR-516b-5p and SOCS2. (G, H) RT-qPCR (G) and western blotting (H) detection (n=3) of the SOCS2 expression upon misexpression of LINC02362. (I) Scatter plot showing the correlation between LINC02362 and SOCS2. (J) Data mining to check the levels of SOCS2 in non-tumor (NT; n=50) or HCC tissues (n=369). (K, L) Kaplan-Meier plots showing the overall survival (OS, K) or disease-free survival (DFS, L) of HCC patients stratified by SOCS expression. ## 0.001 < P < 0.01, *** 0.0001 < P < 0.001, **** P < 0.0001. NS, not significant.