Research Paper Volume 14, Issue 6 pp 2793—2804

Local anesthetic levobupivacaine inhibits stemness of osteosarcoma cells by epigenetically repressing MAFB though reducing KAT5 expression


Figure 3. Levobupivacaine suppresses CSCs properties of osteosarcoma cells. (AD) MG63 and U2OS cells were treated with levobupivacaine (2 mM). (A, B) The stemness was measured by sphere formation assays. (C, D) The protein levels of Sox-2, Oct3/4, and Nanog were analyzed by Western blot. mean ± SD, ** P < 0.01.