Figure 3. Levobupivacaine suppresses CSCs properties of osteosarcoma cells. (A–D) MG63 and U2OS cells were treated with levobupivacaine (2 mM). (A, B) The stemness was measured by sphere formation assays. (C, D) The protein levels of Sox-2, Oct3/4, and Nanog were analyzed by Western blot. mean ± SD, ** P < 0.01.