Research Paper Volume 14, Issue 6 pp 2793—2804

Local anesthetic levobupivacaine inhibits stemness of osteosarcoma cells by epigenetically repressing MAFB though reducing KAT5 expression


Figure 7. Levobupivacaine attenuates cell proliferation and CSCs properties of osteosarcoma cells by regulating KAT5/MAFB. (AE) MG63 and U2OS cells were treated with levobupivacaine, or co-treated with levobupivacaine and KAT5 or MAFB overexpression vectors. (A, B) Cell proliferation was detected by Edu assays. (C, D) Cell apoptosis was analyzed by Annexin V/PI apoptosis detection kit. (E) The stemness was measured by sphere formation assays. mean ± SD, ** P < 0.01.