Research Paper Volume 14, Issue 8 pp 3540—3553

MicroRNA-181a-5p prevents the progression of esophageal squamous cell carcinoma in vivo and in vitro via the MEK1-mediated ERK-MMP signaling pathway


Figure 5. Elevation of miR-181a-5p reduced p-ERK, MMP2 and MMP9 in vivo. Nude mice were injected subcutaneously with ESCC cells and intratumorally injected with miR-181a-5p; (A and B) the protein expression of p-ERK1/2, p-JNK, p-P38, MMP2 and MMP9 as evaluated by Western blotting and RT-qPCR. *P < 0.05 miR-181a-5p mimics vs. NC group; (C) Immunohistochemical staining of MMP9 in miR-181a-5p mimics group and in negative control group; the positive expression rate of MMP9 in miR-181a-5p mimics group and negative control group. *P < 0.05, **P < 0.01 miR-181a-5p mimics vs. NC group.