Research Paper Volume 14, Issue 8 pp 3633—3651

Figure 5. NFκB is involved in the potentiation of IL-6 and TNF-α production by endothelin-1 (ET-1). (A–D) C2C12 and G7 cells were pretreated with NFκB inhibitor PDTC (10 μM) and TPCK (3 μM) then incubated with ET-1 (50 nM) for 24 h. IL-6 and TNF-α levels were examined by RT-qPCR and ELISA. (E–G) C2C12 were incubated with ET-1 for the indicated time intervals, p65, and IKK phosphorylation were examined using Western blotting. Pretreated with NFκB inhibitor PDTC (10 μM), TPCK (3 μM), and Ly294002 then incubated with ET-1 (50 nM) and p65 phosphorylation and IL-6 and TNF-α were examined using Western blotting. Results are expressed of four independent experiments performed in triplicate. ^*p < 0.05 compared with control; ^#p < 0.05 compared with the ET-1–treated group.