Research Paper Volume 14, Issue 9 pp 3921—3940

Figure 3. M2Φ-Exos regulate airway epithelial cell pyroptosis. (A) M2Φ-Exos internalization was observed using a laser scanning confocal microscope. Scale bars = 50 μm. (B) The changes of pyroptosis were observed by electron microscope. Scale bars = 1 μm. (C) The expression level of IL-1was detected by ELISA assay. (n = 3) (D) The relative expression levels of NLRP3 and caspase-1 were determined by qPCR. GAPDH was used as an internal control. (n = 3) (E) The protein expression levels of NLRP3 and caspase-1 were determined by Western blotting. ^*P < 0.05; ^**P < 0.01. Abbreviations: M1Φ-Exos: M1 macrophage-derived exosomes; M2Φ-Exos: M2 macrophage-derived exosomes.