Emergency SARS-CoV-2 variants of concern: rapidly direct RT-qPCR detection without RNA extraction, clinical comparison, cost-effective, and high-throughput

Bing-Heng Yang; Hsing-Yi Chung; Li-Ting Kao; Ming-Jr Jian; Chih-Kai Chang; Jung-Chung Lin; Kuo-Ming Yeh; Chien-Wen Chen; Ya-Sung Yang; Shan-Shan Hsieh; Sheng-Hui Tang; Cherng-Lih Perng; Feng-Yee Chang; Hung-Sheng Shang

doi:doi:10.18632/aging.204095

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COVID-19 Research Paper Volume 14, Issue 11 pp 4624—4633

Emergency SARS-CoV-2 variants of concern: rapidly direct RT-qPCR detection without RNA extraction, clinical comparison, cost-effective, and high-throughput

Figure 2. Correlation between cycle threshold values of N1 (A) and E (B) genes using conventional RT- qPCR and lab-developed RT-qPCR on LC96 or AIO. The correlation coefficient of the analysis on LC96 is R² = 0.9787 for N1 gene and 0.9524 for E gene. The correlation coefficient of the analysis on AIO is R² = 0.9850 of N1 and 0.9798 of E gene.