Research Paper Volume 14, Issue 11 pp 4653—4672

Histone deacetylase 4 reverses cellular senescence via DDIT4 in dermal fibroblasts


Figure 1. HDAC4 is decreased in H2O2- and UV-induced cellular senescence. (A) A scheme of H2O2- and UV-induced cellular senescence using human dermal fibroblasts (B) Senescence-associated beta-galactosidase assay was performed to detect senescent cells for control (left) and senescence-induced (right) cells (n = 4 each). Original magnification: x100. (C) HDAC4 expression analyzed by quantitative RT-PCR or western blotting. Alpha-tubulin was used as loading control. Data represent the mean ± SE (n = 4, ** P <0.01, *** P <0.001). C: control.