Research Paper Volume 14, Issue 11 pp 4653—4672

Histone deacetylase 4 reverses cellular senescence via DDIT4 in dermal fibroblasts


Figure 4. Inhibition of DDIT4 exacerbates cellular senescence. (A, B) Primary dermal fibroblasts were subject to senescence induction with H2O2 or UV. After senescence induction, cells were transfected with negative siRNA or DDIT4 siRNA for 48 h. Beta-galactosidase assay was performed to detect senescent cells. Original magnification: x100. Data represent the mean ± SE (n=3, * P <0.05, ** P <0.01 vs siDDIT4; # P <0.05, ## P <0.01, pCDH vs HDAC4). C: control, NC: scrambled control.