Figure 4. Inhibition of DDIT4 exacerbates cellular senescence. (A, B) Primary dermal fibroblasts were subject to senescence induction with H2O2 or UV. After senescence induction, cells were transfected with negative siRNA or DDIT4 siRNA for 48 h. Beta-galactosidase assay was performed to detect senescent cells. Original magnification: x100. Data represent the mean ± SE (n=3, * P <0.05, ** P <0.01 vs siDDIT4; # P <0.05, ## P <0.01, pCDH vs HDAC4). C: control, NC: scrambled control.