Figure 5. DDIT4 regulates the expression of SASP and age-associated genes. (A) The mRNA levels of senescence-associated secretory phenotype (SASP) and aging-related genes were determined by quantitative real-time PCR. (B) After DDIT4 overexpression, the protein levels of aging-related genes were examined using western blot analysis. Beta-actin served as a loading control. Data represent the mean ± SE (n = 5, ** P <0.01, *** P <0.001).