Figure 6. DDIT4 expression level was decreased in aged human skin in vivo. (A) Buttock skin samples of young and elderly subjects were obtained by punch biopsy. DDIT4 expression in the skin of individuals was examined by immunohistochemistry and quantified. Immunohistochemistry analysis was performed using paraffin-embedded sections. Original magnification: x200. Data represent the mean ± SE (n=6, *** P <0.001 vs Young) (B, C) Quantification of relative mRNA (n = 10) and protein (n = 7) levels of DDIT4 was performed using qPCR and western blotting. GAPDH served as a loading control. Data represent the mean ± SE (** P <0.01 vs Young).