Research Paper Volume 14, Issue 11 pp 4897—4913

Effects of STAT3 on aging-dependent neovascularization impairment following limb ischemia: from bedside to bench


Figure 2. Impaired capillary density, expression of STAT3 and proliferation-associated proteins in young and aged mice post hindlimb ischemia surgery. (A) Representative images and quantification of CD31 immunostaining representing capillary density (red; left panel) and STAT3 (green) expressions in endothelial cells (red; CD 31 positive; right panel). Cell nuclei were stained with DAPI (blue); (B) The quantification of capillary density and (C) STAT3 expression; (D) Representative blots and quantification of PI3K, AKT, STAT3, VEGF, and MMP9 protein expression in the ischemic limbs of young and aged mice. The experiment was repeated in triplicate, *P < 0.05, **P < 0.01, **P < 0.001 and ****P < 0.0001.