Research Paper Volume 14, Issue 11 pp 4897—4913

Effects of STAT3 on aging-dependent neovascularization impairment following limb ischemia: from bedside to bench


Figure 4. Knocking down STAT3 suppresses proliferation, migration and tube formation in human umbilical vein endothelial cells (HUVECs). (A) Cell proliferation, evaluated by measuring BrdU incorporation into cells, (B) cell migration after 24 hours, (C) tube formation, evaluated by the branched points, and (D) phosphorylation protein expression of P-STAT3 and proliferation-associated proteins, including MMP9, VEGF, VEGFR2 and the phosphorylation form of VEGFR2, in HUVECs treated with scramble or STAT3 small interfering RNA (siSTAT3) under normoxia or hypoxia for 24 hours. The experiment was repeated in triplicate, *P < 0.05, ***P < 0.001, and ****P < 0.0001.