Research Paper Volume 14, Issue 13 pp 5390—5405

Figure 3. MIR503HG interacted with SPI1. (A) MIR503HG expression in cytoplasm and nucleus was evaluated in SKOV3 and OVCAR3 cells. U6 was used as a nuclear marker, and GAPDH was used as a cytoplasmic marker. (B) RIP assay was conducted with SPI1 antibody in SKOV3 and OVCAR3 cells, and anti-IgG was used as an internal control. (C) RNA pull-down experiment was performed by using biotinylated MIR503HG transcripts. (D, E) SPI1 mRNA and protein levels were determined after transfection with pcDNA-MIR503HG, MIR503HG siRNA or respective controls. (F) RNAfold web server was used to predict the secondary structure of MIR503HG, and MIR503HG sequence was divided into 5 truncated parts according to its secondary structure. (G) RNA pull-down assay was carried out with biotinylated MIR503HG truncated fragments. ^**P < 0.01. n = 6 in each group. Each test was repeated at least three times independently.