Research Paper Volume 14, Issue 13 pp 5449—5463
Long non-coding RNA H19 contributes to spinal cord ischemia/reperfusion injury through increasing neuronal pyroptosis by miR-181a-5p/HMGB1 axis
Figure 1. SCI/R induces pyroptosis and increases H19 expression level. Mice were subjected to SCI/R or sham surgery. (A) The locomotor function of the hind limbs was investigated by the BMS score in mice after SCI/R for 48 h. (B) Representative histomorphological changes in the ventral horn at 48 h of reperfusion (HE staining). (C) EB fluorescence and neuropathic damage were measured by EB and Nissl staining at 48 h post-injury, respectively. EB (D) and Nissl positive cells (E) were analyzed quantitatively, respectively. The levels of IL-1β (F), IL-18 (G), TNF-α (H) in serum of mice were determined by ELISA assay. (I–L) The protein levels of ASC, Cleaved-caspase-1, and NLRP3 were determined by Western blotting. (M) The expression levels of H19 in the mouse spinal cord tissues following SCI/R. The results were presented as the mean ± SD. N = 10; *P<0.05.