Research Paper Volume 14, Issue 14 pp 5727—5748
The therapeutic effect of Fufang Zhenshu Tiaozhi (FTZ) on osteoclastogenesis and ovariectomized-induced bone loss: evidence from network pharmacology, molecular docking and experimental validation
Figure 7. FTZ suppresses the function of osteoclasts and osteoclast-specific genes expression. (A and B) TRAP staining for osteoclast stimulated by RANKL (Control) and incubated with FTZ-containing serum (FTZ), respectively. (C) The number of the TRAP-stained osteoclasts (>3 nuclei) was calculated. (D and E) Representative images of eroded areas on hydroxyapatite-coated plates under RANKL or FTZ treatments (magnification = 10×). (F) Quantitative analysis of pit area with or with FTZ treatment. (G) BMMs were treated with FTZ for 48 h and the MTS assay was used to measure cell viability. (H–J) Determination of key mRNA expression during osteoclast differentiation by RT-qPCR, including Mmp9, Trap, Nfatc1 (n = 3). *p < 0.05, **p < 0.01.