Research Paper Volume 14, Issue 14 pp 5768—5782

Identification of the ferroptosis-related ceRNA network related to prognosis and tumor immunity for gastric cancer


Figure 4. Relative expression levels of (A) MIR194-2HG, (B) RNF139-AS1, (C) hsa-miR-125b-5p and (D) SLC1A5 in 7 GC cell lines (AGS, MGC-803, SGC-7901, BGC-823, MKN-28, MKN-45, HGC-27) and 1 normal gastric mucosal epithelial cell line (GES-1). The (E) MIR194-2HG expression in SGC-7901 cells, which were transfected with si-MIR194-2HG and control vectors, were validated using quantitative real-time PCR. The effect of MIR194-2HG on the expression levels of (F) hsa-miR-125b-5p and (G) SLC1A5 was examined in SGC-7901 cells with MIR194-2HG knockdown. (H) MIR194-2HG silencing increased the activity of GC cells in vitro by CCK8 assays (*p-value <0.05; # p-value >0.05).