Research Paper Volume 14, Issue 14 pp 5838—5854

Overexpression of 14-3-3ζ primes disease recurrence, metastasis and resistance to chemotherapy by inducing epithelial-mesenchymal transition in NSCLC


Figure 4. 14-3-3ζ promotes NSCLC cells undergoing EMT. (A) Representative images of morphological changes in A549 cells overexpressing 14-3-3ζ. Scale bar = 200 μm. (B) Real time qPCR analysis of EMT biomarkers and transcriptional factors following upregulating 14-3-3ζ in A549 cells (***P < 0.001 vs. EV). (C) Immunoblotting of E-cadherin, vimentin, Snail and Slug protein in the A549 EV and A549 14-3-3ζ cells, respectively. β-actin was used as equal loading control. (D) Immunoblotting of EMT biomarkers in NSCLC cells transfected with indicated siRNA. β-actin was used as equal loading control.