Research Paper Volume 14, Issue 14 pp 5855—5877

Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury


Figure 3. AS-IV protects against renal injury in CLP-induced sepsis. (A) Representative images of H&E staining of tubular epithelial cells of kidney sections obtained at 24 hours after CLP surgery following treatment with low (5 mg/kg) or high dosage (10 mg/kg) AS-IV. (B) Representative images of periodic acid Schiff’s staining reagent depicting basement membrane and brush border in the kidney sections. (C) Semiquantitative analysis of tubular injury. (D) Representative images showing KIM-1 immunohistochemical staining of the apical side of tubular epithelial cells. Serum creatinine (E) and Blood urea nitrogen (F) in mice were measured. (G) Serum IL-6 and IL-1β concentrations were measured by ELISA. Data are presented as the mean ± SEM (n = 6 per group, ***P < 0.001 compared to Sham group, #P < 0.05, ##P < 0.01, ###P < 0.001 compared to CLP group).