Research Paper Volume 14, Issue 14 pp 5878—5894

MBD1/HDAC3-miR-5701-FGFR2 axis promotes the development of gastric cancer


Figure 4. FGFR2 is a direct target of miR-5701. (A) The mRNA levels and protein levels in GC cell lines by qRT-PCR and Western blot. (B) Representative images of FGFR2 in GC tissues by immunohistochemistry. (C) mRNA level and protein level of FGFR2 after miR-5701 overexpression in SGC-7901. (D) miR-5701 is highly conserved across species and has binding sites within the 3′-UTR of human FGFR2; (E) Relative luciferase activity was measured to assess the target relationship of miR-5701 to FGFR2. Data are represented as the mean±SD and experiments were performed in triplicate; * p<0.05; ** p<0.01.