Research Paper Volume 14, Issue 15 pp 6028—6046

Ascorbic acid induces salivary gland function through TET2/acetylcholine receptor signaling in aging SAMP1/Klotho (-/-) mice


Figure 5. The expression of TET2 and DNA methylation status in ascorbic acid-treated SAMP1/kl-/- mice. (A, B) Endogenous TET2 mRNA expression in the salivary glands of SAMP1/kl+/+ and SAMP1/kl-/- mice was measured by RT–PCR or qRT–PCR. GAPDH was used as an internal control. **p < 0.01. (C) The relative expression levels of TET2 protein in the salivary glands between SAMP1/kl +/+ and SAMP1/kl -/- mice were analyzed by Western blotting. (D, E) SAMP1/kl -/- mice (4 weeks old) were randomly divided into two groups (5 mice/group) and orally injected with ascorbic acid (100 mg/kg) or the same volume of saline for 18 days. After treatment, mouse salivary glands were collected and used for the following assays. The expression of TET2 mRNA and protein was determined by qRT–PCR (D) and Western blotting (E). (F) Quantitative analysis of 5-mC levels. Total 5-mC levels in salivary glands isolated from SAMP1/kl -/- mice and SAMP1/kl -/- mice treated with ascorbic acid were measured. *p < 0.05.