Research Paper Volume 14, Issue 15 pp 6028—6046

Ascorbic acid induces salivary gland function through TET2/acetylcholine receptor signaling in aging SAMP1/Klotho (-/-) mice


Figure 7. TET2 knockdown impairs ascorbic acid-mediated salivary gland functional activity. PSGC kl +/+ cells were transfected with siTET2 for 24 hrs. Cells were then treated with ascorbic acid (20 μg/mL) and incubated for 24 hrs, and mRNA expression was evaluated by qRT–PCR. (AD) mRNA expression of M1 AchR, M3AchR, and ADRB1 in PSGC kl +/+ cells treated with ascorbic acid, siRNA TET2, or both. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01. (E) The expression of M1AchR, M3AchR, and ADRB1 protein was determined by Western blotting under the same conditions. (F). Effects of siTET2 on salivary gland functional proteins. PSGC kl +/+ cells were transfected with siTET2 for 48 hrs. After transfection, the protein levels of α-amylase, aqua5, and ZO-1 were analyzed by Western blotting. Actin was used as an internal control.