Research Paper Volume 14, Issue 19 pp 7692—7717

Figure 6. Increased expression of components of NOX complexes and ROS production in M2 macrophages from fibrosis sensitive strains. The expression of ROS producing enzymes (NOX1, NOX2, NOX4) and subunits (NCF1, NCF2, NCF4) of the NOX2 complex was assessed in M2 macrophages polarized by IL-13 treatment. (A) mRNA expression of components of NOX complexes in M2 macrophages was quantified with QPCR and normalized to the expression of β-actin mRNA. (B) The expression of ROS producing enzymes (NOX1, NOX2) and subunits (NCF1, NCF2, NCF4) of the NOX2 complex was determined with western blot. Densitometry was performed for each protein and normalized to actin. Densitometry values are noted below each band. Columns: mean, error bars: +SD, ^*p<0.05 for comparison to the corresponding macrophages treated with vehicle. ^§p<0.05 for comparison to C57L macrophages exposed to IL-13 by ANOVA with Tukey’s correction.