Research Paper Volume 14, Issue 19 pp 7906—7925
Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis
Figure 4. UBE2MP1 transcript sponges miR-145-5p and was positively correlated with RGS3. (A) Predicted binding sequence of UBE2MP1 transcript with the seed sequence of miR-145-5p. The minimum free energy (Mfe) hybridization is calculated as: -21.6 kcal/mol. The comprehensive expression profile of miR-145-5p in pan-cancers (**P<0.001). (B) The expression profile of miR-145-5p in HCC through analysis of the dataset from the TCGA database (**P<0.01). (C) Exploration of the TCGA datasets indicated a significant decline of miR-145-5p in HCC associated with shortened overall survival. (D) The RT-qPCR assay demonstrated a significant decrease of miR-145-5p in three HCC cell lines, in comparison with the control LO2 cells (**P<0.01). (E) The expression of miR-145-5p in both HepG2 and Hep3B cells was significantly elevated by depleting UBE2MP1 (**P<0.01). (F) The dual-luciferase reporter assay verified the direct interaction of UBE2MP1 to miR-145-5p, as a molecular sponge (**P<0.01).