Research Paper Volume 14, Issue 22 pp 8970—8981

Antrodia Camphorata Polysaccharide activates autophagy and regulates NLRP3 degradation to improve liver injury-related inflammatory response


Figure 2. ACP suppressed inflammatory response and NLRP3 activation. (A) NLRP3 fluorescence staining revealed that, LPS promoted NLRP3 expression, and the fluorescence intensity significantly increased, while ACP suppressed NLRP3 expression, significantly lower than LPS group. (BD) Inflammatory factor detection revealed that, ACP decreased the expression of IL-1β, IL-18 and TNF-α, and the difference was significant compared with LPS group. *P<0.05 compared with DMSO, #P<0.05 compared with LPS. (E, F) Protein detection indicated that LC3 expression significantly increased in ACP group, P62 and NLRP3 expression decreased, and NLRP3 expression was negatively correlated with LC3 expression. At the same time, LC3 II expression increased, showing that autophagy was activated. *P<0.05 compared with DMSO, #P<0.05 compared with LPS. (G) Ratio of LC3 I to LC3 II significantly increased when LC3 I was transformed into LC3 II, suggesting that autophagy was activated and the autophagy flow was smooth. *P<0.05 compared with DMSO, #P<0.05 compared with LPS.