Research Paper Volume 14, Issue 21 pp 8615—8632

IGF1 gene therapy in middle-aged female rats delays reproductive senescence through its effects on hypothalamic GnRH and kisspeptin neurons


Figure 4. IGF1 gene therapy effect on the GnRH immunopositive cells. (A) Quantification of GnRH immunopositive cells (NRAd-IGF1 = 10; NPBS = 5). (B). GnRH immunopositive fiber density measured by immunoreactive area (NRAd-IGF1 = 9; NPBS = 5). Error bars represent SD. Two-tailed t-test (A) or Mann Whitney test (B) was used. Asterisks indicate significant (**p < 0.01) differences. (C and D) Immunohistochemistry for GnRH of control (PBS) and experimental (RAd-IGF1) rat’s brain slides. Total magnification of 100× (scale bars: 200 microns).