Research Paper Volume 14, Issue 21 pp 8615—8632

IGF1 gene therapy in middle-aged female rats delays reproductive senescence through its effects on hypothalamic GnRH and kisspeptin neurons


Figure 6. IGF1 gene therapy effect on the Iba1 immunopositive cells. (A and B) Quantification of Iba1 immunopositive cells. Error bars represent SD (Arcuate: NRAd-IGF1 = 8; NPBS = 4. AVPV: NRAd-IGF1 = 7; NPBS = 4). t test with Welch’s correction was used. Asterisks indicate significant (*p < 0.05) differences. Figure 6A post hoc power (1-β) analysis: 0.8211; Figure 6B post hoc power (1-β) analysis: 0.4876. (CF) Immunohistochemistry for Iba1 of control (PBS) and experimental (RAd-IGF1) rat’s brain slides at a magnification of 40× (scale bars: 500 microns), with insets at a magnification of 600× (scale bars: 50 microns). Abbreviation: 3V: third ventricle.