Research Paper Volume 14, Issue 21 pp 8615—8632

IGF1 gene therapy in middle-aged female rats delays reproductive senescence through its effects on hypothalamic GnRH and kisspeptin neurons


Figure 7. IGF1 gene therapy effect on the activation state of microglia. (A and B) Percentage of microglia presenting double immunostaining for Iba1 and MHCII. Error bars represent SD (Arcuate: NRAd-IGF1 = 6; NPBS = 5. AVPV: NRAd-IGF1 = 5; NPBS = 5). Two-tailed t-test was used. Asterisks indicate significant (***p < 0.0005; ****p < 0.0001) differences. Iba1 cells (CF), MHCII cells (GJ) and colocalization of both markers (KN) (Total magnification of 200×; scale bars: 50 microns). Figure 7A post hoc power (1-β) analysis: 0.99997; Figure 7B post hoc power (1-β) analysis: 0.999997.