Research Paper Volume 14, Issue 22 pp 8900—8913

Denervation induces mitochondrial decline and exacerbates lysosome dysfunction in middle-aged mice


Figure 3. Autophagosomal proteins in young and middle-aged control and denervated skeletal muscle. (A) Representative western blots for LC3-I, II and p62 protein. Quantification of (B) LC3-I protein; (C) LC3-II protein; and (D) p62 protein in skeletal muscle. All values were corrected to Ponceau stain (P.S.), and values are reported as means ± SEM, in A.U. *p<0.05, t-test between young and middle-aged control. # represents significance of a 1-way ANOVA on protein levels over the course of denervation in middle-aged mice. *p<0.05, **p<0.01, ***p<0.001 represent post-hoc significance. Trends are represented on graphs. N=12/young and middle-aged control, N=4/denervation time point.