Research Paper Volume 14, Issue 22 pp 8900—8913

Denervation induces mitochondrial decline and exacerbates lysosome dysfunction in middle-aged mice


Figure 6. Lysosome proteins in young and middle-aged control and denervated skeletal muscle. (A) Representative western blots for Lamp1, vATPase, precursor and mature Cathepsin B proteins in skeletal muscle samples. Quantification of (B) Lamp1 protein; (C) vATPase protein; (D) Mature Cathepsin B protein; (E) Precursor Cathepsin B protein, (F) Precursor/total Cathepsin B protein. All values were corrected to Ponceau stain (P.S.), and values are reported as means ± SEM, in A.U. p<0.05, **p<0.01, t-test between young and middle-aged control. # p<0.05, represents significance of a 1-way ANOVA over the course of denervation. *p<0.05, ***p<0.001, ****p<0.0001, post-hoc significance. N=12/young and middle-aged control, N=4/denervation time point.