Research Paper Volume 14, Issue 22 pp 8914—8926

Glutaminase inhibitors rejuvenate human skin via clearance of senescent cells: a study using a mouse/human chimeric model


Figure 1. Increased aging-related markers in aged HDFs. (A) SA-β-Gal-stained image. Bar = 50 μm. (B) Percentage of SA-β-Gal-positive cells. (C) Percentage of BrdU-FITC-positive cells determined using flow cytometry. (D) Comparison of relative gene expression of p16 and p21. *p < 0.05. (E) Comparison of protein expression of p16 and p21. (F) Immunostaining of p16 and p21. Bar = 50 μm. All results are expressed as mean ± SEM. All experiments were independently repeated in triplicate. NS: non-senescence.