Research Paper Volume 15, Issue 8 pp 2877—2890

LINC01296 promotes proliferation of cutaneous malignant melanoma by regulating miR-324-3p/MAPK1 axis

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Figure 2. Knock-down of LINC01296 decreased proliferation abilities of CMM cells. (A) The relative expression of LINC01296 determined by qRT-PCR analysis following the treatment of knocking down LINC01296 (sh-LINC01296) in M21 and A2058 cells. (B) Cell proliferation was examined by CCK-8 assays in sh-LINC01296 group at the indicated time points in M21 and A2058 cells. The sh-NC was as control. (C) Cell proliferation was determined by colony-formation assay of impacts of knocking down LINC01296 in M21 and A2058 cells. (D) EDU assay was used to assess cell proliferation of knocking down LINC01296 in M21 and A2058 cells. All of data were analyzed from three independent experiments. * P < 0.05; ** P < 0.01.