Research Paper Volume 14, Issue 24 pp 9908—9923

Figure 6. Effects of exercise combined with fat body UAS-apoLpp^RNAi on lifespan and age-induced arrhythmias in flies. (A) Arrhythmia index in flies under co-action of exercise with fat body UAS-apoLpp^RNAi. N = 30. Two-way ANOVA was used for LSP2-Gal4>W¹¹¹⁸, LSP2-Gal4>W¹¹¹⁸+exercise, LSP2-Gal4>UAS-apoLpp^RNAi, and LSP2-Gal4>UAS-apoLpp^RNAi+exercise followed by post hoc tests using Bonferroni correction. The P-values for W¹¹¹⁸ and LSP2-Gal4>W¹¹¹⁸+exercise were from student t-tests. (B) The lifespan of flies under co-action of exercise with fat body UAS-apoLpp^RNAi. On the left is the flies′ survival rate (%), and on the right is the median survival of flies. The sample sizes of LSP2-Gal4>W¹¹¹⁸ and LSP2-Gal4>UAS-apoLpp^RNAi+exercise were 205 and 183, respectively. The experiment was carried out with 10 technical replicates and three biological replicates. The P-values for survival curves were obtained from the log-rank test, and the P-values for median survival were obtained from student t-tests. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.