Research Paper Volume 15, Issue 18 pp 9341—9357

Palmitic acid promotes human retinal pigment epithelial cells migration by upregulating miR-222 expression and inhibiting NUMB


Figure 3. Functional miR-222 blockade reversed the palmitic acid promoting effects on RPEC migration. (AD) ARPE-19 cells were transfected with control (NC) or miR-122-specific inhibitors. After 48 h, cells were left untreated, or further treated with 200 μM PA for another 48 h during the indicated assays: (A) Cell viability, (B) wound healing ability, and (C) migration of the indicated ARPE-19 cells. Representative images are shown on left, and the relative wound closure (B) and numbers of invaded cells (C) were summarized. Scale bar, 200 μm. (D) The protein levels of E-cadherin, vimentin, MMP2, and MMP3 in the indicated ARPE-19 cells. Representative bands images are shown, and the relative protein levels were quantitated. n = 3 for each group; *P < 0.05, **P < 0.01. Abbreviation: ns: not significant, between the indicated groups.