Research Paper Volume 15, Issue 8 pp 3021—3034

Figure 2. miR-10a-5p was the target gene of TUSC7. (A) After transfection with TUSC7 overexpression plasmid and shRNA plasmid, q-PCR was used to measure transfection efficiency. (B) Expression of miR-10a-5p after overexpression and silencing of TUSC7 in U251 and U87 cells. (C) Binding site of miR-10a-5p in the TUSC7 transcript. (D) The results of dual-luciferase reporter gene assay showed that the relative luciferase activity was inhibited after co-transfection of wild-type (WT) plasmids and miR-10a-5p mimics, while it was not inhibited after transfection of mutant vector (MUT). ^**p<0.01; ns: p>0.05.