Research Paper Volume 15, Issue 18 pp 9250—9274

High-throughput single-cell profiling of B cell responses following inactivated influenza vaccination in young and older adults


Figure 1. Experimental workflow and subject demographics. (A) PBMC samples were collected from three young and three older adults (midpoint of the age range shown) before and seven days after vaccination. The samples were negatively enriched for B cells. Paired single-cell RNA and B-cell receptor sequencing were then performed. The resulting heavy and light V(D)J sequences were analyzed by immcantation, and gene expression data were analyzed using Seurat. (B) Demographic information of the six subjects. The subjects were selected based on at least 4-fold increase in hemagglutination inhibition titers of at least one vaccine strain at day seven.